Agilent 8453 Diode Array Spectrophotometer
The 8453 UV-Vis spectrophotometer uses a diode array detector to efficiently acquire data across the spectral range in two blocks. The instrument includes both a tungsten and a deuterium lamp, which collectively cover the range from 190 nm to 1100 nm. The instrument includes a standard 1 cm x 1 cm quartz cuvette holder as well as a home-built solid sample holder, constructed for 25 mm cover glass substrates. The typical scan time is 1.5 seconds for the full range, but the instrument can acquire full-range data as quickly as 5 scans per second. Kinetics software is included.
The instrument is located in 1240 Hach Hall. Enquiries should be directed to Dr. Brett Boote. Phone 515-294-5975 (office). Use iLab to reserve the instrument. Reservations do not need to be approved.
FY'26 Rates - Agilent 8453
This instrument is intended for use by students and other researchers. Staff will provide training. The hourly rates are listed below. NOTE: There is a minimum billable increment of one-half hour.
| User Category | Instrument Charge | Staff Assistance |
|---|---|---|
| ISU researchers | $11.00 | $40.00 |
| Non-ISU academic users | $16.50 | $60.00 |
| Non-academic users | $33.00 | $120.00 |
8453 UV-Vis Resources
- Training manual
- Typical QA/QC spectra
- Experimental section and acknowledgements
- Sample submission form (non-ISU users)
Cary 100 Bio Double Beam Spectrophotometer
The Cary 100 Bio system was originally supplied by Varian, but is now part of the Agilent family of instruments. This is a fine double-beam instrument and includes a thermostatted multi-cell transport for automatically analyzing up to 6 samples. The instrument includes both tungsten and deuterium lamps, which collectively cover the range from 190 nm to 900 nm. A full suite of software applications is present, include various kinetics programs, thermal analysis, and a specialized RNA-DNA measurement program. A diffuse reflectance accessory (Harrick Praying Mantis) is also available.
The instrument is located in 1701 Gilman Hall.
FY'20 Rates - Cary 100
This instrument is intended for use by students and other researchers. Staff will provide training. The hourly rates are listed below. NOTE: There is a minimum billable increment of one-half hour. Contact Brett to schedule training or reserve the instrument. Reservations do not need to be approved unless you will need staff assistance.
| User Category | Instrument Charge | Staff Assistance |
|---|---|---|
| ISU researchers | $11.45 | $40.00 |
| Non-ISU academic users | $17.18 | $60.00 |
| Non-academic users | $34.35 | $120.00 |