UV-Vis Spectroscopy

Agilent 3500 Spectrophotometer

a computer sitting next to an instrument on a bench. The instrument looks like a series of white boxes with a lever to open the compartment

The 3500 UV-Vis spectrophotometer is a dual beam instrument with a single channel detector and speeds of up to 15000 nm/min.  The instrument utilizes a xenon flash lamp, which covers the range from 190 nm to 1100 nm.  The instrument is equipped with the Flexible sample compartment for plenty of space mounting solid samples of various types.  The typical scan time is 1.5 seconds for the full range, but the instrument can acquire full-range data as quickly as 5 scans per second.  Kinetics software is included.

The instrument is located in 1240 Hach Hall.  Enquiries should be directed to Dr. Brett Boote.  Phone 515-294-5975 (office).  The instrument currently has no reservations and is walk-up only.

 

FY'26 Rates - Agilent 3500

This instrument is intended for use by students and other researchers.  Staff will provide training.  The hourly rates are listed below.  NOTE: There is a minimum billable increment of one-half hour.

User CategoryInstrument ChargeStaff Assistance
ISU researchers$11.00$40.00
Non-ISU academic users$16.50$60.00
Non-academic users$33.00$120.00

3500 UV-Vis Resources

  • training manual in progress

Cary 100 Bio Double Beam Spectrophotometer

Cary 100 spectrophotometer in a laboratory setting.
Cary 100 Bio Double Beam UV-Vis

The Cary 100 Bio system was originally supplied by Varian, but is now part of the Agilent family of instruments.  This is a fine double-beam instrument and includes a thermostatted multi-cell transport for automatically analyzing up to 6 samples.  The instrument includes both tungsten and deuterium lamps, which collectively cover the range from 190 nm to 900 nm.  A full suite of software applications is present, include various kinetics programs, thermal analysis, and a specialized RNA-DNA measurement program.  A diffuse reflectance accessory (Harrick Praying Mantis) is also available.

The instrument is located in 1238 Hach Hall.

 

 

 

FY'20 Rates - Cary 100

This instrument is intended for use by students and other researchers.  Staff will provide training.  The hourly rates are listed below.  NOTE: There is a minimum billable increment of one-half hour.  Contact Brett to schedule training or reserve the instrument.  Reservations do not need to be approved unless you will need staff assistance.

User CategoryInstrument ChargeStaff Assistance
ISU researchers$11.45$40.00
Non-ISU academic users$17.18$60.00
Non-academic users$34.35$120.00

 

Cary 100 UV-Vis Resources