Fluorescence Instruments

Horiba DeltaFlex Time Correlated Single Photon Counting Fluorometer

The Horiba DeltaFlex is a time-correlated single photon counting (TCSPC) system capable of measuring lifetimes of fluorescence and phosphorescence excited states on the sub-ns to greater than 1 second time scales. The system is composed of LED sources, a monochromator, and photomultiplier tube capable of detection from 300-850 nm. The main types of measurements associated with this setup:

  • Fluorescence/Phosphorescence Lifetimes: Time-correlated excited state decays for liquid/solvated and solid samples.
  • Anisotropy: Motorized polarizers allow for collection of parallel and perpendicular decays, from which sum, difference, and the G-factor are calculated. 
  • Time-Resolved Emission Spectra (TRES): After collection of several wavelength-dependent lifetime decays, the overall decay profile is sliced into three sections and the decay-associated spectra are determined. Up to 5 linked lifetimes can be used to describe the data set.  

The instrument is located in 1240 Hach Hall.  Enquiries should be directed to Dr. Brett Boote. Phone 515-294-5975 (Hach office).

Horiba TCSPC Resources


Perkin Elmer LS 55 Fluorescence Spectrometer

The Perkin Elmer LS 55 is a research grade luminescence spectrometer capable of acquiring excitation, emission, and synchronous spectra, as well as concentration determination and kinetics studies at fixed wavelengths. The xenon lamp can be turned off for chemiluminescence measurements as well. Samples can be excited from 200-800 nm and data can be collected from 200-900 nm. This instrument is much more sensitive than the Horiba TCSPC when collecting luminescence spectra due to the brighter xenon lamp. It also collects spectra at a faster rate. 

A front surface accessory for solid sample analysis is available as well, built for samples center-mounted on 1" substrates. 

The instrument is located in 1240 Hach Hall.  Enquiries should be directed to Dr. Brett Boote. Phone 515-294-5975 (Hach office).


Perkin Elmer LS55 Resources


Rates FY'20

These instruments are intended to be used by students and other researchers. Staff will provide training and some initial methods development assistance, and will configure the instrument and assist with special projects as required.  The hourly rates are listed below.  NOTE:  There is a minimum billable increment of thirty minutes.

User Category Instrument Charge Staff Assistance
ISU researchers $11.00 $30
Non-ISU academic users $16.50 $45
Non-academic users $33.00 $90