QA-QC Protocols and Spectra

ESI tuning and calibration of the Agilent QTOF 6540 is achieved using the Agilent test mix (table). This tune mix is always present in bottle B under the ion source.



The system is put  in the ON state: In the Context pull down, select Tune.

Select calibrant bottle B for the calibrant mixture to flow into the ion source. The flow stream from LC should go to waste. Select polarity. Make sure all the calibrant masses are selected.




Verify that the resolution of mass 922.0097 (Positive ion mode) mass 1033.9881 (negative ion mode) is about 40,000 (FWHM).


The deviation across the mass range should be under 1 ppm.