QA-QC Protocols - Agilent 8453 UV-Vis
Proper instrument performance can normally be verified by running:
- System blank
- Holmium oxide glass standard
- "Camphor" aqueous solution
For critical work, allow the instrument and the lamps to stabilize for at least twenty minutes. The blank is run with nothing in the sample compartment.
Holmium Oxide Glass Standard
This standard is kept in the drawer. Please do NOT take this precious standard!
Camphor Aqueous Solution
This is the same standard used to verify CD performance with the Jasco J-710 spectrophotometer. The solution is made by dissolving 60.0 mg of ammonium-d-10-camphorsulfonate in distilled water. Distilled water (10 mm quartz cuvette) should be run as the reference.