Experimental Section Template and Acknowledgments
Acknowledgement of NSF Support - Instrument Purchase
"Purchase of the ThermoFinnigan LCQ Advantage mass spectrometer used to obtain results included in this publication was supported by the National Science Foundation under MRI Grant No. CHE ?????.
"Any opinions, findings, and conclusions or recommendations expressed in this material are those of the author(s) and do not necessarily reflect the views of the National Science Foundation."
Acknowledgement of CIF Staff Assistance
"We wish to thank ISU Chemical Instrumentation Facility staff members <insert name(s) here> for training and assistance pertaining to the LCQ Advantage LC/MS results included in this publication."
"Experimental Section" Information
Flow Injection ESI:
"Positive (and/or negative) ion mode mass spectra were obtained using the LCQ Advantage mass spectrometer. Surveyor Pump system , equipped with Surveyor autosampler was used. 1 ul of sample (concentration of approximately 10 ppm) was injected into the LCQ Advantage ESI ion source. Water/MeOH (.1% Formic acid) 50/50 was used as effluent solvent. The mass range was kept constant from 100 to 1000 amu. The instrument was operated in the 4GHz HRes mode. Accurate mass measurement was achieved by constantly infusing a calibrant (masses: 121.0508 and 922.0098)."
Complete experimental conditions can be found in the Qual browser.
HPLC/ESI: ESI experimental conditions are same as indicated above.
LC conditions are as follows:
LC column: Agilent XDB C18, 4.6*150 mm, 1.8 um
Flow rate: 800 ul/min
Column temperature: 30 deg
UV wavelength: 254 nm
Injection volume: 1 ul
Gradient LC: 90% Solvent A (H2O, 1% Formic acid) to 70% Solvent B (ACN, .1% Formic acid) in 10 min. 70% B was maintained for 1 min then 90% A was reestablished.