Experimental Section Information (BMT Sterivap)
A Sterivap model HP 446 (Bruno, Czechoslovakia) was used for the high-pressure steam-sterilization of <liquids, wastes, containers, tools....> used in this research. The sterilization program parameters were <choose from the method parameters shown in the table below>:
| Pgrm | Name | Sterilization Temp (C) | Sterilization Time (min) | Number of Evacs | Cooling Temp (C) | Number of Drying Phases | Drying Phase Length (min) |
| P2 | Universal | 134 | 7 | 4 | 2 | 2 | |
| P3 | Universal Containers | 134 | 7 | 4 | 4 | 3 | |
| P4 | Rubber | 121 | 20 | 4 | 1 | 4 | |
| P7 | Tools Fast | 134 | 4 | 1 | 1 | 3 | |
| P8 | Liquids | 121 | 20 | 1 | 95 | 0 | 0 |
| P11 | 121 C - 15 min media | 121 | 15 | 4 | 0 | 0 |
NOTE: The paper-tape printout for your sterilization run will also contain all of the pertinent information.
Experimental Section Information (Primus PSS 500)
A Primus model PSS500 (Omaha, NE) was used for the high-pressure steam-sterilization of <liquids, wastes, containers, tools....> used in this research. The sterilization program parameters were <choose from the method parameters shown in the table below>:
| Parameter | Gravity Pgrm | Vacuum Pgrm | Liquids Pgrm | Vacuum - Biohazard |
| Final ramp slope (deg / min) | 5.0 | 5.0 | 5.0 | 5.0 |
| Half ramp time (mm:ss) | 0:30 | 0:30 | 0:40 | 0:30 |
| # of postvacs | 1 | 1 | 1.0 | 1 |
| Dry vac point (inches Hg) | -20.0 | -20.0 | 0.1 | -20.0 |
| # of pre-vacs | 0 | 3 | 0 | 3 |
| Prevac vac time (mm:ss) | 0:30 | 0:30 | 0:30 | 0:30 |
| Prevac vac point (inches Hg) | -20.0 | -20.0 | -20.0 | -20.0 |
| Purge time (mm:ss) | 1:30 | 1:30 | 2:00 | 1:30 |
| Precharge pressure (psig) | 12.0 | 12.0 | 12.0 | 12.0 |
| Dry time (mm:ss) | 2:00 | 2:00 | 8:00 | 2:00 |
| Sterilize temp (C) | 132.1 | 132.1 | 121.1 | 136 |
| Sterilize time (mm:ss) | 3:30 | 3:30 | 30:00 | 120:00 |
NOTE: The paper-tape printout for your sterilization run will also contain all of the pertinent information.