UV-Vis spectroscopy
Two UV-Vis instruments are available including the Agilent 8453 Diode Array Spectrophotometer and the Cary 100 Bio Double Beam Spectrophotometer.
Agilent 8453 Diode Array UV-Vis
Summary - Agilent 8453 UV-Vis
The 8453 UV-Vis spectrophotometer uses a diode array detector to efficiently acquire data across the spectral range in two blocks. The instrument includes both a tungsten and a deuterium lamp which collectively cover the range from 190 nm to 1100 nm. The instrument includes a standard 1 cm x 1 cm quartz cuvette holder as well as a home-built solid sample holder, constructed for 25 mm cover glass substrates. The typical scan time is 1.5 seconds for the full range, but the instrument can acquire full range data as quickly as 5 scans per second. Kinetics software is included.
The instrument is located in 1240 Hach Hall. Enquiries should be directed to Dr. Brett Boote. Phone 515-294-5975 (office). Use iLab to reserve the instrument. Reservations do not need to be approved.
FY'20 Rates - Agilent 8453
This instrument is intended for use by students and other researchers. Staff will provide training. The hourly rates are listed below. NOTE: There is a minimum billable increment of one-half hour.
| User Category | Instrument Charge | Staff Assistance |
|---|---|---|
| ISU researchers | $10.30 | $30 |
| Non-ISU academic users | $15.45 | $45 |
| Non-academic users | $30.90 | $90 |
8453 UV-Vis Resources
- Training manual
- Typical QA/QC spectra
- Experimental section and acknowledgements
- Sample submission form (non-ISU users)
Cary 100 Bio Double Beam UV-Vis
Summary - Cary 100 Bio UV-Vis
The Cary 100 Bio system was originally supplied by Varian, but is now part of the Agilent family of instruments. This is a fine double-beam instrument and includes a thermostatted multi-cell transport for automatically analyzing up to 6 samples. The instrument includes both tungsten and deuterium lamps which collectively cover the range from 190 nm to 900 nm. A full suite of software applications is present, include various kinetics programs, thermal analysis, and a specialized RNA-DNA measurement program. A diffuse reflectance accessory (Harrick Praying Mantis) is also available.
The instrument is located in 1701 Gilman Hall.
FY'20 Rates - Cary 100
This instrument is intended for use by students and other researchers. Staff will provide training. The hourly rates are listed below. NOTE: There is a minimum billable increment of one-half hour. Contact Brett to schedule training or reserve the instrument. Reservations do not need to be approved unless you will need staff assistance.
| User Category | Instrument Charge | Staff Assistance |
| ISU researchers | $11.45 | $30 |
| Non-ISU academic users | $17.18 | $45 |
| Non-academic users | $34.35 | $90 |
Cary 100 UV-Vis Resources