UV-Vis spectroscopy

Two UV-Vis instruments are available, including the Agilent 8453 Diode Array Spectrophotometer, and the Cary 100 Bio Double Beam Spectrophotometer.

Agilent 8453 Diode Array UV-Vis                                                        Cary 100 Bio Double Beam UV-Vis

   

 

Summary - Agilent 8453 UV-Vis

The 8453 UV-Vis spectrophotometer uses a diode array detector to efficiently acquire data across the spectral range in two blocks.  The instrument includes both a tungsten and a deuterium lamp which collectively cover the range from 190 nm  to 1100 nm.  The instrument includes a standard 1 cm x 1 cm quartz cuvette holder.  The typical scan time is1.5 seconds for the full range, but the instrument can acquire full range data as quickly as 5 scans per second.  Kinetics software is included.

The instrument is located in 1240 Hach Hall.  Enquiries should be directed to Steve Veysey.  Phone 515-294-5805 (office).  Use "Booked" to reserve teh instrument.  Reservations do not need to be approved.

FY'18 Rates - Agilent 8453

This instrument is intended for use by students and other researchers.  Staff will provide training.  The hourly rates are listed below.  NOTE: There is a minimum billable increment of one-half hour.

User Category Instrument Charge Staff Assistance
ISU researchers $10.30 $30
Non-ISU academic users $15.45 $45
Non-academic users $30.90 $90

 

8453 UV-Vis Resources

 

Summary - Cary 100 Bio UV-Vis

The Cary 100 Bio system was originally supplied by Varian, but is now part of the Agilent family of instruments.  This is a fine double-beam instrument and includes a thermostatted multi-cell transport for automatically analyzing up to 6 samples.  The instrument includes both tungsten and deuterium lamps which collectively cover the range from 190 nm  to 900 nm.  A full suite of software applications is present, include various kinetics programs, thermal analysis, and a specialized RNA-DNA measurement program.  A diffuse reflectance accessory (Harrick Praying Mantis) is also available.

FY'18 Rates - Cary 100

This instrument is intended for use by students and other researchers.  Staff will provide training.  The hourly rates are listed below.  NOTE: There is a minimum billable increment of one-half hour.  Use "Booked" to reserve the instrument.  Reservations do not need to be approved unless you will need staff assistance.

User Category Instrument Charge Staff Assistance
ISU researchers $11.45 $30
Non-ISU academic users $17.18 $45
Non-academic users $34.35 $90

 

Cary 100 UV-Vis Resources