Mass Spectrometry

The Mass Spectrometry Lab, located in 1238 Hach Hall, is committed to providing excellent Mass Spectrometry services including teaching. The laboratory provides mass spectrometry analysis of a wide range of chemicals. Among those species are synthetic organic and inorganic compounds, plant extracts and their metabolites, and compounds of biological interest such as peptides and proteins et cetera.

In addition to discussing the available instrumentation and services, this website is intended to help under-graduate, graduate students, and researchers acquire background and expertise in mass spectrometry. Researchers are being trained to use GC/MS and /or LC/MS instruments and are allowed to use them 24/7. Comments or suggestions are welcome.

Please contact: Dr. Kamel Harrata, kamel@iastate.eduPhone: 515-294-4392 (office), 515-294-5544 (lab).

Current instrumentation include: (Video Description)

  • LC-MSMS-Accurate Mass: Agilent 6540 QTOF (Video) equipped with 1200 series U-HPLC system including autosampler and UV detector.  Available for customer use and sample submission.  With a resolving power of greater than 40,000 and a mass range greater than 3000 Daltons (singly charged species), this instrument routinely provides mass measurements with an accuracy better than 2 parts-per-million.
  • LC-MSMS: Thermo LCQ ion-trap based mass spectrometer with Surveyor HPLC system including autosampler and UV detector.  This instrument is archived, but can be made available for long-term special projects.
  • LCMS: Shimadzu LCMS2020 (Video) quadrupole-based nominal mass system with complete LC system including autosampler and V detector.  Configured for "walk-on" customer use; also avaialble for long-term special projects.
  • GCMS-Accurate Mass-Pyrolysis: Waters GCT (Video) time-of-flight based mass spectrometer with Agilent 6540 GC and autosampler.  This instrument has a resolving power in excess of 7000, and typically provides mass measurements accurate to within 5-10 ppm.  This is a customer-operated instrument.  It is normally configured for Pyrolysis GCMS measurements.  Assistance with special projects is available.
  • GCMS: Agilent 5973 GCMS quadrupole based system with Agilent 6540 GC, ChemStation acquisition software, and MassHunter processing software. This is a customer-operated instrument.