AC-200 Operating Instructions

1. Be Sure the Instrument is Set Up for the Nucleus Desired.

This instrument is not computer switchable.  For all nuclei except proton, both the probe
tune/match and preamp values must be the same as those written on the sheet attached to the
magnet stand. Be sure to wipe the spinner and sample with a Kimwipe before placing the
sample in the magnet..

2. Place the Sample in the Magnet.

a) Remove the cap from the top of the magnet.
b) Push the Orange button and the Lift button on the AMOS panel (to the right of the
    keyboard).  Listen for the lift air.
c) Float the tube/spinner on top of the magnet.
d) Push the Lift-Off button.  Sample should sink down into the magnet.

3. Lock the Magnetic Field on the Deuterium Signal.

a) You should see a lock signal of some kind moving across the monitor. Using ^L as needed
    (^L is a 3 position toggle: lock only/lock plus data/data only).
b) Push LOCK POWER and adjust it using the black knob. ( ~30 for CDCl3, 15 for
    Acetone, and  20 for everything else.)
c) Push FIELD and use the knob to center the lock signal. (CDCL3 is at ~3675,  D2O at
    ~3630, acetone at ~3585). The two triangles should be spaced equally on each side of
    center.
d) Push LOCK.  When the light is on (not flashing) the magnetic field is locked. Be noted that:
        1) You may need to increase the LOCK GAIN for the system to lock.
        2) It may be of helpful to move the field slightly to obtain the lock.
        3) Push Dual Sweep to turn it off if you were using it.

4. Shim, Using Z and Z2.

a) First, adjust LOCK GAIN so that the lock signal is between the top two grid marks.
b) Shim using Z and Z2 by pushing the button and adjusting the knob.  Repeat the preceding
    adjustment for maximum lock level. You may change the LOCK GAIN accordingly to
    keep the lock signal visible.
c) Push Stand-By when satisfied.

5. Entering Commands.

a) Most commands must be followed by a <Return>, with the exception being in EP mode.
b) <Del> can be used to delete the last character typed.
c) ^O will delete the whole line.
d) If the instrument is not responding, type ^Q.  Rebooting the computer is not recommended
    unless absolutely necessary.
e) Do not use <Bs> to erase mistakes!!!

6. Setting up the Spectrometer.

Read in a standard file (RJ filename.ext <Return>). You have two choices:
     1. standard files for carbon, phosphorous, and proton are (Please ask for other nuclei):
           P31:5.CDCL  P31:10.CDCL C13:5.CDCL  C13:10.CDCL H1.CDCL
           P31:5.D2O   P31:10.D2O  C13:5.D2O   C13:10.D2O  H1.D2O
           P31:5.ACET  P31:10.ACET C13:5.ACET  C13:10.ACET H1.ACET
      2. An alternative to RJ is RE filename.ext <Return> of an old file.
 
7. Type II<Return> to Set up Parameters.

Be sure that probe tune/match and the 3 preamp knobs are set.

8. Collecting Data.

a) Type  RGA <Return> and wait for this command to execute.
b) The standard files (except proton) have NS set to -1 so that the spectrometer will collect
    data until you stop it.  You may change NS by typing NS <Return> and entering the
    number of scans desired, followed by a <Return>.
c) Type ZG<Return> to begin data acquisition. Scans are counted in the upper left of the
    screen. ^H or <Return> will stop the data acquisition.

9. Saving Data.

a) If desired, type TI<Return> sample description <Return>.
b) Type WR filename.ext <Return>. Filename can be up to 8 characters, the extension is 3
    digits.

10. Looking at the Spectrum During Acquisition.

a) The data system is organized into 3 independent blocks (Jobs 1, 2, 3).
b) The number showed on the screen is the block you are currently in. This can be changed
    simply by typing the number of the block you wish to be in.
c) You can only transfer the contents of the block you are currently in. Transfer is initiated by
    typing TR<Return>, and followed by  entering the number of the block you wish the data
    to be transferred to. You can only transfer the data to a block other than the one where
    acquisition is occurring! A <Return> will transfer the data to the block requested.
d) Example:  If you are collecting data in block 1 and want to transfer it to block 2, type a 1 if
    you are not currently in this block, and then type TR; <Return>; 2; <Return>.
e) When the data has been transferred out of the acquisition block, it may be processed as
    described in section 11.
f) Typing ^H or Bs will stop the acquisition.
 
11. Processing Data.

a) Type LB<Return> and enter the desired number followed by a <Return>. Usually select
    0.1 for proton and 1 for carbon. The minimum value allowed is 0.01.
b) Type EF<Return> or EFP<Return>.
c) Type EP<Return>. This command enters the expand and phase mode in which phasing,
    chemical shift, plot limits, and peak heights can be set.
      1. A <Return> will exit the EP mode.
      2. ^R displays the entire spectrum, ^B allows the use of A and B knobs
      3  Phasing
          a) Turn the A knob (left or right shift) until the biggest peak is displayed.
          b) Type P
          c) Turn the C knob to phase the peak, type ^C to reverse the sense of this knob if you
              reach the end.
          d) The vertical display  buttons (to the left of the keyboard) may be used to control the
              height  and the B knob  the width of the peak.
          e) Turn the A knob until a peak remote from the biggest peak is displayed.
          f)  Turn the D knob to phase this peak, type ^D to reverse the sense of this knob if you
              reach the end.
          g) Turn the A knob to return to the biggest peak and repeat steps c-g if necessary.
          h) Type M to memorize the phase.
      4. Setting the reference.
          a) Turn the A knob to locate the reference peak.
          b) Use the C knob to place the cursor on top of this peak. The D knob is fine control of
              the cursor.
          c) Type Gxx<Return> where xx is the chemical shift.
       5.    <Return> exits EP mode.

12  Transferring the Data to Xwinnmr.

a)  In an unused job, type RE filename.ext <Return>
b)  type AU SEND.AU<Return>
 
13. Leaving the Instrument.

a) Type PO<Return> to turn off the decoupler.
b) Push <Return> to turn the Lock off.
c) Push the Orange Button and the Sample lift button to eject sample.
d) Push Lift off to turn off eject air.
e) Put cap on top of magnet.
f) Log off CIA.

14. Process the Data on nmr or nmrtwo - the SGI UXNMR Processing Computers.

a) Log-onto nmr or nmrtwo as usual, and start xwinnmr.
b) Type btran<Return>
c) First time users must select  Edit Program Options. The options should be:
        /dat2/bruknet   (Current Destination)
        ac200             (Current station)
        bruker             (Current user)
        dat2                (disk)
        your username (owner)
d) Click SAVE
e) Select Select, Convert (no renaming), YourFile, Conv, Quit
f) The data should be in your directory now.

15. Plotting from the AC-200.

a) Be sure that the plotter and parallel converter are plugged in,  and paper is loaded.
b) Enter the plot limits in the ep mode. Type ep<Return> F xx<Return> yy<Return> and a
    final <Return> to exit EP. Where xx is the left chemical shift and yy is the right chemical
    shift.
c) Type DPO<Return> to set the plot options you want. This only has to be done once for
    one block.
d) Type TOPL<Return> and wait for the command to execute
e) Type PX<Return> and wait for the command to execute
f)  Type NP<Return> and a plot should eventually appear.